The Kernel CSFV E^rns Ab is a double-antigen ELISA. The microtiter plate is coated with recombinant CSFV E^rns antigen. During sample incubation, antibodies to the CSFV E^rns protein bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies to the CSFV E^rns protein are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620-650 nm) is optional. The OD value correlates to the concentration of the antibodies to the CSFV E^rns protein in the sample.

Kernel CSFV E^rns Ab is for detection of antibodies to the E^rns protein of the classical swine fever virus (CSFV) in porcine serum and plasma samples. 

The Kit is suitable as a CSFV screening test. DIVA potential: The Kernel CSFV E^rns Ab enables differentiation between infected and appropriate CSFV marker-vaccinated animals (e.g., Suvaxyn^® CSFV marker).